Cell-Derived Plasma Membrane Vesicles Are Permeable to Hydrophilic Macromolecules

Halt Proliferation and Induce Differentiation Human Plasma Membrane-Derived Vesicles

Biologically active Fas antigen and its cognate ligand are expressed on plasma membrane-derived extracellular vesicles.

Exfoliation of plasma membrane components is a directed process that consumes energy and requires active cell metabolism. Proteins involved in regulating the survival and proliferation of eukaryotic cells are released on exfoliated vesicles. We examine here whether the Fas receptor and its cognate ligand (FasL) are present on vesicles shed from high metastatic potential CX-1 cells and low metas...

A New Approach to Provide High Water Permeable Polyethersulfone based Nanofiltration Membrane by Air Plasma Treatment

In this study, polyethersulfone based nanofiltration membranes were modified by air plasma generated through dielectric barrier discharge to increase the membrane hydrophilicity aiming to improve the separation and antifouling characteristics. The effect of plasma time on the physico-chemical and separation properties of membrane was investigated. The PES nanofiltration membranes were fabricate...

plasma-membrane vesicles

Transport of [14C]tetraethylammonium (TEA), an organic cation, was studied in brush-border (BBMV) and basolateral (BLMV) membrane vesicles isolated from rabbit kidney cortex. In BBMV, the presence of an outwardly directed H+ gradient induced a marked stimulation of TEA uptake against its concentration gradient (overshoot phenomenon), whereas a valinomycin-induced inside-negative potential had n...

Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles.

Cell-derived GPMVs (giant plasma-membrane vesicles) enable investigation of lipid phase separation in a system with appropriate biological complexity under physiological conditions, and in the present study were used to investigate the cholesterol-dependence of domain formation and stability. The cholesterol level is directly related to the abundance of the liquid-ordered phase fraction, which ...